The M5226 mouse anesthesia mask has a conical design for the breathing port, which can fit the mouse's head tightly and minimize the leakage of anesthetic gas into the surrounding environment, which not only improves the utilization efficiency of anesthetic gas, but also reduces the concentration of anesthetic gas in the environment, ensuring the safety of experimenters. The mask can be installed on the M5268 anesthesia surgery board to effectively fix the mouse's body and prevent it from moving during the experiment, thereby ensuring the smooth progress of the experimental operation.