The M5225 rat anesthesia mask has a conical design for the breathing port, which can fit the rat's head tightly and minimize the leakage of anesthetic gas into the surrounding environment, which not only improves the utilization efficiency of anesthetic gas, but also reduces the concentration of anesthetic gas in the environment, ensuring the safety of experimenters. The mask can be installed on the M5268 anesthesia surgical board to effectively fix the body of the mouse and prevent it from moving during the experiment, thereby ensuring the smooth progress of the experimental operation.